CD4⁺ T cells account for approximately 30-45% of the CD3⁺ T cell population. They are commonly isolated from peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation, fluorescence-activated cell sorting (FACS), or by immunomagnetic bead-based selection. Following isolation, T cells can be expanded in cell culture media containing cytokines such as IL-2, or IL-7 and IL-15. Additional cytokine combinations are used to drive the differentiation of specific CD4⁺ T helper cell subsets in vitro.

As cytokines are some of the most important components of T cell culture media, they need to display high levels of activity, purity, and lot-to-lot consistency. Our stringent production and purification standards, coupled with our rigorous in-house testing ensure our proteins will provide robust T cell expansion and differentiation with minimal variability from one experiment to the next. Our catalog includes research-grade, Animal-free Preclinical, and GMP-grade proteins to meet the needs of all scientists from basic research to clinical manufacturing.

* Animal-free Preclinical and GMP-grade proteins are available for these molecules.

† Liquid formulations of the GMP and Animal-free Preclinical grades of these proteins are available.