High-throughput environments, such as automated manufacturing lines and quality control laboratories, require fast, reliable endotoxin testing to match the pace of operations. In many cases, a detection threshold of 0.1 EU/mL is sufficient. However, while some assays offer greater sensitivity, they often require extended incubation times, creating delays in decision-making and workflow efficiency. This application note demonstrates that the PyroGene® Recombinant Factor C (rFC) Endpoint Fluorescent Assay can be effectively adapted to a 20-minute incubation protocol while maintaining reliable detection at 0.1 EU/mL. Despite a reduced assay time, it continues to deliver accurate, precise, and reproducible results across multiple reagent lots and detection systems. This validated, sustainable approach to endotoxin testing is well-suited for laboratories that prioritize speed and efficiency without sacrificing per.

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