PYROGENT Gel clot assay is a qualitative LAL test for Gram-negative bacterial endotoxin with sensitivies of 0.03, 0.06, 0.125 and 0.250 EU/ml. It is an excellent choice for laboratories testing limited number of samples. It is also an economical choice in that no major equipment purchases are necessary. LAL, as supplied, is reconstituted with LAL reagent water and then mixed in equal parts with the samples solution being tested. After a 1-hour incubation, the samples are read visually by simply invert the reaction tube. In the presence of endotoxin, gelation occurs; in the absence of endotoxin, gelation does not occur. (top page)

PYROGENT-5000is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent, placed in an incubating plate reader and automatically monitored over time for the appearance of turbidity. PYROGENT-5000 is measured at the 340 nm wavelength and has sensitivity range from 0.01 to 100 EU/ml. PROGENT-5000 is perfect for laboratories needing to process large numbers of samples, large volume parenterals and water rinses from medical devices.
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Kinetic-QCL is aquantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. Kinetic-QCL is the most sensitive of the LAL assays, having a sensitivity range of < 0.005 EU/ml-50 EU/ml. A sample is mixed with the LAL/Substrate reagent, placed in an incubating plate reader and monitored over time for the appearance of a yellow color. It is the chromogenic nature of the Kinetic-QCL assay that makes it the most appropriate choice for testing small volume parenterals, vaccines, antibiotics and biologicals. Kinetic-QCL is less affected by inhibitory products products that may interfere with the clotting mechanism in turbidimetric and gel-clot assays. (top page)

Products :
Endotoxin Detection Assays
- Pyrogent Recombinant Factor C assay Beyond
- Pyrogent Gel Clot Assays
- Pyrogent-5000 Kinetic Turbidimetria Assay
- QCL-1000 Chromogenic LAL Endotoxin Assay
- Kinetic-QCL Kinetic Chromogenic Assay

NEW Endotoxin Removal Products

Endotrapo Endotoxin Removal Products

Instrumentation and Software
- ELx808csc Plate Reader
- On-site Service and Preventive Maintenance Agreements
- Standard LAL Kinetic System
- FLx800 Fluorescent Plate Reader
- WinKQCL Software v.2.00 and Manuals
- WinKQCL Software v.3.00,21 CFR Part II Compliant and Manuals

Accessory Products
- Endotoxin ( E.coli ) Stardard
- Endotoxin ( E.coli ) Challenge vials
- LAL Reagent Grande Multi-well Plates
- B-Glucan Blocker
- Pyrosperse Dispersing Agent
- MgC12, 10 mM Solution
- Tris Buffer, 50 mM Solution
- Pyrogent-free test tubes
- Reagent Reservoir
- LAL Reagent Water
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The Limulus Amebocyte Lysate ( LAL ) assay is used to detect endotoxin associated with Gram negative bacteria. The lysate is prepared from the circulating amebocytes of the horsehoe crab ( Limulus polyphemus )

There are four LAL methodologies currently licensed by the FDA. The first, referred to as gel-clot method is based on the fact that LAL clots in the presence of endotoxin. The kinetic turbidimetric method is a quantitative LAL method that used the appearance turbidity to determine endotoxin content. The third and fourth methodologies, referred to as the chromogenic assaysemploy a synthetic chromogenic substrate that, in the presence of LAL and endotoxin, produces a yellow color that is linearly related to the endotoxin concentration.

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