Spheroids are advantageous given their simplicity, shorter culture timeline, and relatively low cost compared to organoids.5 3D spheroid cultures surpass the capability of their 2D culture counterparts particularly in drug screening.2,4 For example, tumor-derived spheroids are comprised of tumor cell types and mimic the physical tumor properties, allowing for a direct study of the tumor microenvironment.2,4,5 Because spheroids are relatively simple, one of their limitations is the lack of polarity and self-organization into higher order tissue structures, so they don’t always represent the in vivo environment.